We make your cell cultures more ALIVE
Our solution is a simple in vitro culture system that mimics key in vivo conditions
In Research
Our mission is to provide equipment for the study of cells in physiologically relevant conditions. We aim to have this equipment successfully used by a diverse group of researchers.
In Drug Development
Our mission is to impact human health by improving candidate drug selection, reducing animal testing, and increasing clinical trial success rates.

Simple



Media Circulation



Customizable



The VitroFlo Approach
The VitroFlo system is comprised of a microfluidic cell culture plate and a rocker mechanism. Variations in the culture plate channel geometry, membrane porosity, and channel spacing support a variety of models and cell types. The programmable rocker mechanism can achieve a variety of media flow conditions and fluid shear rates.
VitroFlo Culture Plate
The VitroFlo patent-pending culture plate contains paired media reservoirs connected by one or more microfluidic channels to create a recirculating flow cell. The culture plates are compatible with manual and automated liquid handling and imaging systems.






Media Circulation with VitroFlo
Rocking the VitroFlo plate creates flow in one direction through the microfluidic cell culture channels and flow in the other direction through bypass channels. The results are controlled, near-continuous unidirectional flow and shear rates for cultured cells. This delivers nutrients, removes waste, and stimulates cells.
Cell Co-Culture
The VitroFlo plate has a second microfluidic channel that is separated from the flow channel by a thin porous membrane. This channel can be used to culture a second cell type or monitor transport across the cells lining the upper channel. By combining two cell culture channels with a variety of fluidic conditions, the VitroFlo system can be used for a wide range of applications. We are proud of the work being done by our users and we are actively looking for new partners.



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VitroFlo Options



2D Flow



2D/2D Co-Culture



2D/3D Co-Culture
VitroFlo can be used in several mono and co-culture configurations. Applications include modeling barrier function, biochemical signalling, drug absorption, and more. With no pumps or tubing and the ability to run 12 independent tests in a single wellplate, the VitroFlo is sure to accelerate you to your research goals.
2D Flow Wellplates



channel area | 49 mm2 |
channel height | 286 µm |
cover layer (culture surface) | glass |
wellplate material | polystyrene |
2D/2D Co-Culture Wellplates



channel area (Upper) | 49 mm2 |
channel height (Upper) | 286 µm |
channel area (Lower) | 125 mm2 |
channel height (Lower) | 286 um |
cover layer (culture surface) | glass |
wellplate material | polystyrene |
2D/3D Co-Culture Wellplates



channel area (Upper) | 49 mm2 |
channel height (Upper) | 286 µm |
channel area (Lower) | 125 mm2 |
channel height (Lower) | 286 um |
cover layer (culture surface) | glass |
wellplate material | polystyrene |
VitroFlo Rocker
Position 1: perfusion flow, 1dynes/cm2 shear, 200µL/min flow
Position 2: mild shear, 3dynes/cm2 shear, 600µL/min flow
Position 3: medium shear, 6dynes/cm2 shear, 1100µL/min flow
Position 4, high shear, 11dynes/cm2 shear, 2000µL/min flow
The Rocker can be operated inside an incubator without a PC connection.


